Author: Daniel Zadory
Stereology: Probing for Precision
Stereology is a tool for obtaining unbiased estimates of object numbers and/or sizes within three-dimensional regions of interest. For biological samples, quantitative measurements are potentially more objective and accurate when compared to traditional semi-quantitative grading of findings by pathologists (i.e., scoring of morphologic changes as minimal, mild, moderate, or severe). Although both approaches require high […]
Immunostaining Assay Optimization – Tackling Intrinsic Study Variables
Immunohistochemistry or Immunofluorescence (IHC/IF) is employed routinely for investigative and diagnostic evaluations of antigen-antibody interactions within cells or tissues. While most IHC/IF target antigens are proteins, other antigenic biological substrates can also be recognized by antibodies (e.g., carbohydrates, lipids, or nucleic acids). Accurate and reproducible results are achieved when procedures used to identify and localize […]
How to Ensure the Quickest Possible Histology Project Timeline
With the processing of up to 50,000 tissues per month, and projects regularly flowing in and out of our labs, histology scheduling at EPL is a continual process. Recognizing that the project timeline, in addition to quality and expertise, is one of the most important factors to our clients, we’re providing the following tips on the items […]